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ISSN: 2095-3119
CN: 10-1039/S
e-ISSN: 2352-3425

Vacuolar metabolomic and proteomic profiling reveals the vacuole composition of ripe juice sacs and functions of CsTST2 and CsERDL6 in sugar accumulation in citrus

•An efficient protocol was established to isolate intact vacuoles from ripe juice sacs of four citrus varieties.•Vacuole multi-omics analysis identified 640 metabolites and 1,782 proteins, and pummelo...

Establishment of gene expression and silencing systems in Echinacea purpurea

•Optimized experimental parameters for the Agrobacterium tumefaciens-mediated transient expression and virus-induced gene silencing systems in Echinacea purpurea leaves.•Established gene expression...

CmERFV-2 regulates CmCBF3 and CmMYB44 to inhibit sucrose accumulation in oriental melon fruit at low temperature

•Low temperature reduces sucrose accumulation in oriental melon fruit by suppressing ethylene production.•CmCBF3 repressed ethylene biosynthesis in melon fruit under low temperature via suppressing...

Varietal traits for achieving high yield, superior quality, and strong lodging resistance in both inbred and hybrid rice in Central China

•High-quality hybrid rice achieved higher yields than inbred rice due to increased panicle size.•Hybrid and inbred rice had comparable grain quality and lodging resistance.•Key traits associated with...

Feasibility of using X-ray to irradiate adult for control of the oriental fruit moth, Grapholita molesta in orchards

•Confirming that irradiation of adults induces sterility and consequently reduces population size.•The sub-sterilizing dose for male adults (1-day-old) was 130 Gy, resulting in a sterility rate of 76.34%.•Pairing...

Natural variation in NIN-LIKE PROTEIN 4 is associated with divergent nitrogen use efficiency in Brassica juncea

•Identifies a high-NUE genotype H158 with superior antioxidant capacity, nitrogen assimilation, and uptake efficiency.•Reveals a key gene BjuB04.NLP4, whose G/C variant influences transcriptional regulation...

Whole-genome resequencing of 391 Chinese donkeys reveals population structure and provides insights into their morphological and adaptive traits

•A total of 127, 117, and 169 selective-signal genes related to coat color, body size, and high-altitude adaptation were identified based on 391 whole-genome sequencing (WGS) data of Chinese donkeys.•By...

Brassinosteroid signaling drives floral transition defects in Camellia sinensis ‘Ziyang 1’ via CsBZR2-mediated suppression of CsFLC

•The non-flowering tea plant line ZY1H provides a unique germplasm resource for exploring floral induction mechanisms.•CsBZR2-mediated repression of CsFLC and altered brassinosteroid signaling are implicated...

Accumulation of beneficial haplotypes in the Huang-Huai-Hai wheat region and its application in molecular breeding

•The Huang-Huai-Hai wheat region (HHHR) shows superior yields and disease resistance, and beneficial haplotypes are more frequent than in other regions.•MFP-a gene may affect both grain development...

Plastic film mulching increases maize yields and mitigates NH3 emissions under climate change and N deposition

•Plastic film mulching (PFM) reduced NH3 emissions by lowering the pH, increasing water contents, and exerting barrier effects.•Daily NH3 emissions under PFM were only driven by soil NH4 content.•NH3...

Identification and functional characterization of GmMACPF1 as a negative regulator of salt tolerance during germination

•Five elite salt-tolerant soybean germplasms were identified from 165 mutant lines, and 60 single nucleotide polymorphisms and five novel quantitative trait loci associated with salt tolerance were...

Silver nanoparticles as a multifunctional nanotechnology for green control of fungal stress and microplastic-associated heavy metal toxicity in soybean

•Silver nanoparticles (Ag-NPs) suppress fungal pathogens under microplastic (MP)-contaminated conditions•MP-driven heavy metal accumulation is reduced by Ag-NPs•Ag-NPs activate antioxidant and defense...

An mRNA vaccine co-expressing IFN-α-GP5-IgG Fc fusion protein and M protein protects piglets against highly pathogenic PRRSV challenge

An mRNA vaccine (IGF+rM) encoding modified GP5 and M proteins of HP-PRRSV HuN4 was successfully prepared.The IGF+rM mRNA vaccine elicited partial cellular immune responses and antibody production in...

Metagenomic and phylogeographic analyses reveal the evolutionary dynamics and epidemic ecology of Porcine Circoviruses in China

1.Nationwide mNGS survey reveals PCV2d dominance and frequent PCV co-infections2.Nationwide co-circulation and recombination heighten the risk of PCV emergence3.PCV3 in China dates to 2002; Hebei is...

Multi-variety rice yield prediction from heterogeneous UAV time-series data via a cross-period attention transformer

Pioneering yield prediction from multi-year, multi-variety heterogeneous UAV time-series data.The riceCPAT, an improved cross-period attention Transformer, achieves the best accuracy (R2=74%) with fewer...

Improved canopy structure and post-silking productivity contribute to higher grain yield of densely planted summer maize

•Smaller leaf angles above the ear were associated with more favorable canopy light distribution in high-yielding populations.•Sustained leaf function after silking was associated with greater dry matter...

Novel highly potent and environmentally benign molluscicides for Pomacea canaliculata control: Discovery, mechanism, and field validation

•Novel D-1 displays excellent molluscicidal activity against invasive Pomacea canaliculata.•20% D-1 suspension achieves over 85% snail control in large-scale UAV rice field trials.•D-1 shows minimal...

Proton-responsive SlSTOP1–SlFRDL1 regulatory pathway modulates citrate-driven iron acquisition in tomato roots

•Iron deficiency induces organic acids and proton exudation to solubilize sparingly available iron.•Citric acid exhibits superior iron solubilization efficacy at weakly acidic pH.•SlSTOP1 binds to the...

PDCoV nsp14 interferes with the interferon pathway by degradin MAVS, MyD88, and TRAF3 proteins via the autophagy and proteasome pathways

•Porcine deltacoronavirus (PDCoV) nsp14 inhibits interferon (IFN) expression.•PDCoV nsp14 degrades MAVS, MyD88 and TRAF3 proteins via the MARCH8-NDP52-autophagosome and proteasome pathways.•Host proteins...

The Clygp (yellow-green plant) encodes a signal recognition particle 54 kDa protein modulating chloroplast development and photosynthesis in watermelon

•A novel chlorophyll-deficient yellow-green mutant PKH352 was identified from an EMS-induced watermelon mutant library.•Mutation in ClG42_04g0106300 gene, encoding a signal recognition particle 54 kDa...

Integrated hormone and transcriptome analyses of Chinese cabbage mutant lic86 reveal the involvement of auxin in leaf curling

•Chinese cabbage leaf inward curling mutant, lic86, displayed markedly increased transverse leaf curvature.•Exogenous IAA induced outward leaf curling, and treatment with the auxin transport inhibitor...

Fertilizer-N recovery links to SOC stabilization and fractionation in straw-mulched soil: Insights from 15N-tracing and 13C natural abundance

•1% macroaggregate rise boosts SOC stock by 0.17 Mg ha−1 and N recovery by 0.41%.•Fertilizer-N retention in aggregates is associated with increased SOC stabilization.•Stable SOC exhibited strongest...

Distinct decomposition dynamics of heterogeneous carbon components in cultivated agricultural soils controlled by flexible microbial substrate utilization strategy

•Plant debris dominates SOC mineralization, and microbial necromass controls stability.•A shift from bacteria to fungi drives carbohydrates-to-lignin decomposition in C-rich soil.•Actinomycetes dominate...

A novel fucosylation-specific cell wall-degrading enzyme promotes Magnaporthe oryzae infection

•The α-L-fucosidase MoFco1 specifically hydrolyzes XXFG, an α-1,2-fucosylated xyloglucan oligosaccharide derived from plant hemicellulose.•MoFco1 enzymatic activity is crucial for the full virulence...

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